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Depositing Lab
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Depositing OrganizationMemorial Sloan-Kettering Cancer Center
Located in United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20969 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 20969-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 20969-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHistone cluster 1, H2bb
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Alt nameH2B.1
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SpeciesH. sapiens (human)
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Insert Size (bp)400
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GenBank IDNM_021062
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Entrez GeneH2BC3 (a.k.a. H2B.1, H2B/f, H2BFF, HIST1H2BB)
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Tag
/ Fusion Protein
- Orange (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer Sp6
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The accession number for H2B.1 sequence X57127 was obtained from Fraser et al., 2005 (https://doi.org/10.1002/gene.20139) The actual DNA was PCR amplified from HEK293 genomic DNA then cloned into pEGFP-N1 as described in Kanda et al., 1998 (https://doi.org/10.1016/s0960-9822(98)70156-3) That H2B.1-EGFP construct was used as template to synthesize the construct here. An EcoRI site in the H2B.1 sequence was silently mutated. The Orange cDNA was obtained from Dr. Roger Tsien.
DNA (Catalog # 20969-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 20969-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
H2B-Orange was a gift from Robert Benezra (Addgene plasmid # 20969 ; http://n2t.net/addgene:20969 ; RRID:Addgene_20969) -
For your References section:
High levels of Id1 expression define B1 type adult neural stem cells. Nam HS, Benezra R. Cell Stem Cell. 2009 Nov 6. 5(5):515-26. 10.1016/j.stem.2009.08.017 PubMed 19896442
