RCAS(B)-Rheb-Q64L
(Plasmid #21050)

• Depositing Lab: Peter Vogt
• Publication: Jiang et al Oncogene. 2008 Sep 25. 27(43):5729-40.

Backbone

• Vector backbone: pBR322
• Backbone size w/o insert (bp): 11689
• Vector type: Bacterial Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ras-homolog enriched in brain
• Alt name: Rheb
• Species: H. sapiens (human)
• Insert Size (bp): 685
• Mutation: glutamine 64 to leucine
• GenBank ID: AF493921
• Entrez Gene: RHEB (a.k.a. RHEB2)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SfiI(A) (not destroyed)
• 3′ cloning site: SfiI(B) (not destroyed)
• 5′ sequencing primer: AGCTCCGCGGGCCACCATGGACTACAAGGATGACGATGACAAGGCGGCCGCGCCGCAGTCCAAG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The creation of RCAS(B)-SfiI and the adapter plasmid pBSFI was described in Aoki et al., PNAS. 1998 Dec 8;95(25):14950-5. The SacII/XbaI fragment was cloned into pBSFI and the Sfi fragment was further cloned into RCAS(B)-SfiI.