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pCAG-hHDAC6
(Plasmid #21099)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21099 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 21099-D50 50 μg of DNA in Tris buffer $495
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DNA 21099-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCAGEN
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Histone Deacetylase 6
  • Alt name
    HDAC6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3400
  • Entrez Gene
    HDAC6 (a.k.a. CPBHM, HD6, JM21, KDAC6, PPP1R90)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XHO1 (not destroyed)
  • 3′ cloning site NOT1 (not destroyed)
  • 5′ sequencing primer PCAG-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    J.R. Lu, University of Florida

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

encoding wild type HDAC6 protein with degenerate codons

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-hHDAC6 was a gift from Connie Cepko (Addgene plasmid # 21099 ; http://n2t.net/addgene:21099 ; RRID:Addgene_21099)

  • For your References section:

    HDAC4 regulates neuronal survival in normal and diseased retinas. Chen B, Cepko CL. Science. 2009 Jan 9. 323(5911):256-9. 10.1126/science.1166226 PubMed 19131628
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