|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13813||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.1 +
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
MutationTranscript variant 2
Entrez GeneSIRT2 (a.k.a. SIR2, SIR2L, SIR2L2)
/ Fusion Protein
- flag (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Cloned into a version of pcDNA3.1 that has been modified to include a C-terminal Flag tag (see author's map for details).
This variant (2) lacks the additional 47 nt present in the 5' end of the coding region in transcript variant 1. Translation is thought to initiate at a downstream in-frame ATG.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SIRT2 Flag was a gift from Eric Verdin (Addgene plasmid # 13813 ; http://n2t.net/addgene:13813 ; RRID:Addgene_13813)
For your References section:The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin deacetylase. North BJ, Marshall BL, Borra MT, Denu JM, Verdin E. Mol Cell. 2003 Feb . 11(2):437-44. 10.1016/S1097-2765(03)00038-8 PubMed 12620231