pRPA70-IRES-Ven-RPA32-IRES-RPA14
(Plasmid #211430)

• Purpose: mammalian expression of human RPA32 N-terminally fused to mVenus and RPA70 and RPA14
• Depositing Lab: Helmut Pospiech
• Depositing Organization: Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)
  Located in Germany
• Publication: Pospiech Lab Plasmids 2023 (unpublished)

Backbone

• Vector backbone: pmVenus-C1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RPA70 - IRES - RPA32 - IRES - RPA14
• Alt name: RPA1 and RPA2 and RPA3
• Species: H. sapiens (human)
• Entrez Gene: RPA1 (a.k.a. HSSB, MST075, PFBMFT6, REPA1, RF-A, RP-A, RPA70)
• Entrez Gene: RPA2 (a.k.a. REPA2, RP-A p32, RP-A p34, RPA32)
• Entrez Gene: RPA3 (a.k.a. REPA3, RP-A p14)
• Tag / Fusion Protein:
  • mVenus (on RPA32) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (destroyed during cloning)
• 3′ cloning site: XbaI (not destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

generated by Julia Kutz (Leibniz Institute on Aging - Fritz Lipmann Institute, D-07745 Jena, Germany)

DNA (Catalog # 211430-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 211430-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.