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SA-betageo
(Plasmid #21709)

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Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21709 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 21709-D50 50 μg of DNA in Tris buffer $495
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DNA 21709-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pBluescript KS (-)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Mouse gene trapping
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SAbgeo
  • Alt name
    bifunctional lacZ/neomycin phosphotransferase gene
  • Species
    bgeo fusion protein

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pSAbgeo, gene trap vector, use this as a source of SA for targeting ROSA26.

For more information visit -
http://research.mssm.edu/soriano/lab

We recommend you order this plasmid along with pROSA26-1, pROSA26-5' as a "ROSA26 targeting kit".

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SA-betageo was a gift from Philippe Soriano (Addgene plasmid # 21709 ; http://n2t.net/addgene:21709 ; RRID:Addgene_21709)

  • For your References section:

    Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice. Friedrich G, Soriano P. Genes Dev. 1991 Sep . 5(9):1513-23. 10.1101/gad.5.9.1513 PubMed 1653172
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