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Depositing Lab
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Depositing OrganizationNYU Grossman School of Medicine
Located in United States of America -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21898 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 21898-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 21898-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGL3 and pGL2 modified
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4310
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Vector typeNon-viral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl2
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Growth instructionsStbl2 30C
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCHOP
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SpeciesM. musculus (mouse)
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Insert Size (bp)9300
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Entrez GeneDdit3 (a.k.a. AltDDIT3, CHOP-10, CHOP10, chop, gadd153)
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer EBV-R
- 3′ sequencing primer EGFP-N
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This reporter gene closely tracks the expression of endogenous CHOP.
DNA (Catalog # 21898-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 21898-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CHOP::GFP was a gift from David Ron (Addgene plasmid # 21898 ; http://n2t.net/addgene:21898 ; RRID:Addgene_21898) -
For your References section:
Feedback inhibition of the unfolded protein response by GADD34-mediated dephosphorylation of eIF2alpha. Novoa I, Zeng H, Harding HP, Ron D. J Cell Biol. 2001 May 28. 153(5):1011-22. 10.1083/jcb.153.5.1011 PubMed 11381086
Map uploaded by the depositor.
