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phGluc
(Plasmid #22522)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22522 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 22522-D50 50 μg of DNA in Tris buffer $495
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DNA 22522-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    ph2m
  • Backbone size w/o insert (bp) 3906
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gaussia luciferase
  • Alt name
    Gaussia princeps luciferase
  • Alt name
    GLuc
  • Species
    Gaussia princeps
  • Insert Size (bp)
    557

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Snab1 (destroyed during cloning)
  • 3′ cloning site NheI (destroyed during cloning)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GenBank Style annotations can be found at:
http://home.ccr.cancer.gov/LCO/target.htm

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phGluc was a gift from Christopher Buck (Addgene plasmid # 22522 ; http://n2t.net/addgene:22522 ; RRID:Addgene_22522)

  • For your References section:

    Quantitation of human seroresponsiveness to Merkel cell polyomavirus. Pastrana DV, Tolstov YL, Becker JC, Moore PS, Chang Y, Buck CB. PLoS Pathog. 2009 Sep . 5(9):e1000578. 10.1371/journal.ppat.1000578 PubMed 19750217
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