pJB036
(Plasmid
#22624)
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Depositing Lab
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Depositing OrganizationNational Institute of Neurological Disorders and Strokes (NINDS)
Located in United States of America -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22624 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 22624-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 22624-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonep18CAT
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Backbone manufacturerR. McKinnon
- Backbone size w/o insert (bp) 4728
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Vector typeCAT reporter plasmid
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5 alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehuman genomic PLP promoter region
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SpeciesH. sapiens (human)
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Insert Size (bp)4200
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GenBank IDNG_008863
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Entrez GenePLP1 (a.k.a. GPM6C, HLD1, MMPL, PLP, PLP/DM20, PMD, SPG2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert is 4200bp (approx) of the Human PLP 5' upstream, promoter region and Exon1 (-4200 to +85) in the correct orientation. Insert is a BamHI/HindIII from pJB010
This PLP DNA cloned by Hudson lab.
DNA (Catalog # 22624-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 22624-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJB036 was a gift from Lynn Hudson (Addgene plasmid # 22624 ; http://n2t.net/addgene:22624 ; RRID:Addgene_22624) -
For your References section:
Identification of cis-regulatory elements in the myelin proteolipid protein (PLP) gene. Berndt JA, Kim JG, Hudson LD. J Biol Chem. 1992 Jul 25. 267(21):14730-7. PubMed 1378839