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pAc5.1B-EGFP-DmAGO1
(Plasmid #22768)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22768 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 22768-D50 50 μg of DNA in Tris buffer $495
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DNA 22768-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pAc5.1B-EGFP
  • Backbone manufacturer
    Elisa Izaurralde
  • Backbone size w/o insert (bp) 6063
  • Vector type
    Drosophila expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Dh5 alpha and XL1-blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Argonaut-1, variant A
  • Alt name
    AGO1
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    2955
  • GenBank ID
    NM_166020
  • Entrez Gene
    AGO1 (a.k.a. Dmel_CG6671, AGO-1, Ago, Ago-1, Ago1, Agp1, CG6671, Dm Ago1, DmAgo1, Dmel\CG6671, MRE20, ago-1, ago1, ago1-1, anon-WO0257455.29, argonaute1, dAGO1, dAgo1, dmAgo1, l(2)04845, l(2)4845, l(2)k00208, l(2)k08121)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer Ac5 forward primer site
  • 3′ sequencing primer BGH reverse priming site
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1B-EGFP-DmAGO1 was a gift from Elisa Izaurralde (Addgene plasmid # 22768 ; http://n2t.net/addgene:22768 ; RRID:Addgene_22768)

  • For your References section:

    GW182 interaction with Argonaute is essential for miRNA-mediated translational repression and mRNA decay. Eulalio A, Huntzinger E, Izaurralde E. Nat Struct Mol Biol. 2008 Apr . 15(4):346-53. 10.1038/nsmb.1405 PubMed 18345015
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