Skip to main content
Addgene
Open Menu

HPRT
(Plasmid #22884)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22884 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 22884-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 22884-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pBluescript SK (-)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 9000
  • Vector type
    Cre/Lox ; homologous recombination

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    hypoxanthine phosphoribosyltransferase-1
  • Alt name
    HPRT; HGPRT; HPRT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    11900
  • Mutation
    PGK, NeoR, and BGH polyA are between the two genomic sequences (one section is 9.1kb and one is 2.5kb).
  • GenBank ID
    NC_000023
  • Entrez Gene
    HPRT1 (a.k.a. HGPRT, HPRT)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI and XmaI (not destroyed)
  • 3′ cloning site EcoRI and NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer pLac
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that very little of the insert has been sequenced by Addgene. Please be sure to do your own sequencing of HPRT before using this plasmid.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HPRT was a gift from James Thomson (Addgene plasmid # 22884 ; http://n2t.net/addgene:22884 ; RRID:Addgene_22884)

  • For your References section:

    Homologous recombination in human embryonic stem cells. Zwaka TP, Thomson JA. Nat Biotechnol. 2003 Mar . 21(3):319-21. 10.1038/nbt788 PubMed 12577066
Related items:
Commonly requested with: