pCIG-VP16 RARa [TJ#389]
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16287||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6200
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameRAR alpha
Alt nameretinoic acid receptor, alpha
SpeciesH. sapiens (human)
Insert Size (bp)2300
Entrez GeneRARA (a.k.a. NR1B1, RAR)
/ Fusion Protein
- VP16 (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
VP16-RARalpha. Note that this contains the 3'UTR of RARalpha, though no polyA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCIG-VP16 RARa [TJ#389] was a gift from Thomas Jessell (Addgene plasmid # 16287 ; http://n2t.net/addgene:16287 ; RRID:Addgene_16287)
For your References section:A requirement for retinoic acid-mediated transcriptional activation in ventral neural patterning and motor neuron specification. Novitch BG, Wichterle H, Jessell TM, Sockanathan S. Neuron. 2003 Sep 25. 40(1):81-95. 10.1016/j.neuron.2003.08.006 PubMed 14527435
Map uploaded by the depositor.