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pSV Sport RXR alpha
(Plasmid #8882)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8882 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 8882-D50 50 μg of DNA in Tris buffer $495
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DNA 8882-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pSV-Sport
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3160
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RXR alpha
  • Alt name
    RXRa
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1866
  • Entrez Gene
    RXRA (a.k.a. NR2B1, RXR-alpha, RXRalpha)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    cDNA for hRXRa from Dr. Ron Evans (Mangelsdorf et al. 1990 Nature 345 p224)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSV Sport RXR alpha was a gift from Bruce Spiegelman (Addgene plasmid # 8882 ; http://n2t.net/addgene:8882 ; RRID:Addgene_8882)

  • For your References section:

    mPPAR gamma 2: tissue-specific regulator of an adipocyte enhancer. Tontonoz P, Hu E, Graves RA, Budavari AI, Spiegelman BM. Genes Dev. 1994 May 15. 8(10):1224-34. 10.1101/gad.8.10.1224 PubMed 7926726
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