UbG76V-eGFP-V5His_15aa
(Plasmid #23969)

• Depositing Lab: Nico Dantuma
• Depositing Organization: Karolinska Institutet
  Located in Sweden
• Publication: Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.
• Citations: 1 Article

Backbone

• Vector backbone: EGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3970
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ub-G76V-eGFP-V5HIS_15aa
• Alt name: Ubiquitin
• Alt name: UBB
• Alt name: Ub-g76v-eGFP
• Species: H. sapiens (human)
• Insert Size (bp): 990
• Mutation: G76V mutation in Ubiquitin. Ub fused to N-terminus of EGFP. Extended C-terminal peptide sequence after EGFP containing first 15aa of a modified V5HIS tag
• GenBank ID: NM_018955
• Entrez Gene: UBB (a.k.a. HEL-S-50)
• Tag / Fusion Protein:
  • eGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid does not express a functional V5-His tag. This deletion construct was used to determine the minimum peptide length of a C-terminal tag to allow degradation of UbG76V in cells depleted of p97. The translated fusion protein ends with the following sequence: SCRPL ESRGP FEGKP*

For more information, see the associated publication.

DNA (Catalog # 23969-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 23969-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.