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pTreTight-Htt94Q-CFP
(Plasmid #23966)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 23966 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTRE-tight
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 2605
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    N-terminal fragment Huntingtin Q94
  • Alt name
    Htt
  • Alt name
    HttQ94
  • Alt name
    Htt polyQ
  • Species
    H. sapiens (human)
  • Mutation
    94 polyQ repeat
  • Entrez Gene
    HTT (a.k.a. HD, IT15, LOMARS)
  • Tag / Fusion Protein
    • CFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

There are 96Q repeats and an Argenine in the middle of the repeat.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTreTight-Htt94Q-CFP was a gift from Nico Dantuma (Addgene plasmid # 23966 ; http://n2t.net/addgene:23966 ; RRID:Addgene_23966)
  • For your References section:

    Accumulation of ubiquitin conjugates in a polyglutamine disease model occurs without global ubiquitin/proteasome system impairment. Maynard CJ, Boettcher C, Ortega Z, Smith R, Florea BI, Diaz-Hernandez M, Brundin P, Overkleeft HS, Li JY, Lucas JJ, Dantuma NP. Proc Natl Acad Sci U S A. 2009 Aug 18. 106(33):13986-91. 10.1073/pnas.0906463106 PubMed 19666572