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pT7-PE7-pA.v3
(Plasmid #241028)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241028 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pT7
  • Modifications to backbone
    Plasmid contains T7 promoter modified for use with CleanCap AG reagent, a 3' UTR, a 120 pA-tail and a BpiI/BtgZI site to allow run-off transcription
  • Vector type
    In vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PE7
  • Species
    Synthetic
  • Insert Size (bp)
    6912
  • GenBank ID
  • Promoter T7

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-PE7-pA.v3 was a gift from Jacob Giehm Mikkelsen (Addgene plasmid # 241028 ; http://n2t.net/addgene:241028 ; RRID:Addgene_241028)

  • For your References section:

    Prime editing of human hematopoietic stem cells for correction of GATA2 deficiency. Wolff JH, Skov TW, Dorset SR, Sjelborg J, Ravendran S, Revenfeld ALS, Haslund D, Moller AM, Holm M, Thomsen MK, Mogensen TH, Moller BK, Bak RO, Mikkelsen JG. Sci Transl Med. 2026 Sep 30;18(869):eaec7031. doi: 10.1126/scitranslmed.aec7031. Epub 2026 Sep 30. 10.1126/scitranslmed.aec7031 PubMed 42814803
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