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pI12
(Plasmid #24273)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24273 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5409
  • Vector type
    Mammalian Expression ; T7 for in vitro transcription
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    modified adenovirus exons and introns
  • Alt name
    splicing reporter
  • Species
    Modified from adenovirus
  • Insert Size (bp)
    246

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is heavily modified. Use our sequence and map rather than on based on pcDNA3. For example, HindIII and EcoRI are switched from pcDNA3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pI12 was a gift from Mariano Garcia-Blanco (Addgene plasmid # 24273 ; http://n2t.net/addgene:24273 ; RRID:Addgene_24273)
  • For your References section:

    A stem structure in fibroblast growth factor receptor 2 transcripts mediates cell-type-specific splicing by approximating intronic control elements. Baraniak AP, Lasda EL, Wagner EJ, Garcia-Blanco MA. Mol Cell Biol. 2003 Dec . 23(24):9327-37. 10.1128/MCB.23.24.9327-9337.2003 PubMed 14645542