Purpose(Empty Backbone) enables eukaryotic expression of N-terminally FLAG-HA-tagged fusion proteins
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||52535||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||52535-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5501
Modifications to backboneaddded/inserted sequences encoding "FLAG–5xGly–HA–5xGly" tag at start of MCS to allow for fusion protein production
Vector typeMammalian Expression
- Promoter CMV
Selectable markersNeomycin (select with G418)
/ Fusion Protein
- FLAG-HA (N terminal on backbone)
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer BGH poly reverse (Common Sequencing Primers)
Information for Cloning Grade DNA (Catalog # 52535-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FLAG-HA-pcDNA3.1- was a gift from Adam Antebi (Addgene plasmid # 52535 ; http://n2t.net/addgene:52535 ; RRID:Addgene_52535)
For your References section:DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. elegans Developmental Timing and Maturation. Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M, Antebi A. Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 10.1016/j.devcel.2014.01.028. 10.1016/j.devcel.2014.01.028 PubMed 24613396