pEGFP-N1-FLAG
(Plasmid #60360)

• Purpose: (Empty Backbone) Plasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.
• Depositing Labs: Sébastien Britton, Patrick Calsou
• Publication: Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):9047-62. doi: 10.1093/nar/gku601. Epub 2014 Jul 16.
• Citations: 34 Articles

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size (bp): 4733
• Modifications to backbone: Insertion of a FLAG tag in front of GFP together with an ATG and a strong kozak sequence.
• Vector type: Mammalian Expression
• Promoter: CMV
• Selectable markers: Neomycin (select with G418)
• Tags / Fusion Proteins:
  • FLAG (N terminal on backbone)
  • EGFP (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry