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DNA damage triggers SAF-A and RNA biogenesis factors exclusion from chromatin coupled to R-loops removal.

Britton S, Dernoncourt E, Delteil C, Froment C, Schiltz O, Salles B, Frit P, Calsou P
Nucleic Acids Res. 2014 Oct 1;42(14):9047-62. doi: 10.1093/nar/gku601. Epub 2014 Jul 16. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
60360pEGFP-N1-FLAGPlasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.
60362pEGFP-N1-FUS/TLS-FLAGCPlasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal tag). Confers resistance to G418.
60364pICE-NLS-mCherryPlasmid for expression of NLS-mCherry (control plasmid). Confers resistance to Puromycin. Expression is inducible in T-REx cells.
60365pICE-RNaseHI-WT-NLS-mCherryPlasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
60367pICE-RNaseHI-D10R-E48R-NLS-mCherryPlasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.

Antibodies from Article