pcDNA3-Ro2
(Plasmid #24503)

• Depositing Lab: Bruce Conklin
• Depositing Organization: J. David Gladstone Institutes
  Located in the United States of America
• Publication: Coward et al Proc Natl Acad Sci U S A. 1998 Jan 6;95(1):352-7.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RASSL Ro2
• Alt name: Ro2
• Alt name: KOR
• Species: H. sapiens (human)
• Mutation: mutations in extracellular loops 2 (see comments below) and 3 (E297Q), making it a RASSL.
• Entrez Gene: OPRK1 (a.k.a. K-OR-1, KOP, KOR, KOR-1, KOR1, OPRK)
• Tags / Fusion Proteins:
  • FLAG (N terminal on insert)
  • NLS from prolactin (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI/XhoI (not destroyed)
• 3′ cloning site: XbaI (destroyed during cloning)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Extracellular Loop 2 (EL2) mutations shown in lowercase: aSSVGISAIVLGvTqpRdgavvCtLQFPspswyWDtvt

Prolactin NLS sequence: MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS

For more information about this plasmid see: http://openwetware.org/wiki/R02-ORF

DNA (Catalog # 24503-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 24503-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.