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pIRES2-eGFP-Ofd1-Myc-G139S
(Plasmid #24581)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24581 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pIRES2-eGFP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5578
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ofd1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3087
  • Mutation
    Glycine 139 changed to serine (codon GGT to AGT)
  • GenBank ID
    NM_177429
  • Entrez Gene
    Ofd1 (a.k.a. ORF2, Cxorf5, DXGgc7e)
  • Tags / Fusion Proteins
    • Myc (C terminal on insert)
    • eGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer ccctaggtccccagagaca
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutation reported in:

Clinical, molecular, and genotype-phenotype correlation studies from 25 cases of oral-facial-digital syndrome type 1: a French and Belgian collaborative study.

Thauvin-Robinet C, Cossée M, Cormier-Daire V, Van Maldergem L, Toutain A, Alembik Y, Bieth E, Layet V, Parent P, David A, Goldenberg A, Mortier G, Héron D, Sagot P, Bouvier AM, Huet F, Cusin V, Donzel A, Devys D, Teyssier JR, Faivre L.

J Med Genet. 2006 Jan;43(1):54-61.PMID: 16397067

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES2-eGFP-Ofd1-Myc-G139S was a gift from Jeremy Reiter (Addgene plasmid # 24581 ; http://n2t.net/addgene:24581 ; RRID:Addgene_24581)
  • For your References section:

    Ofd1, a human disease gene, regulates the length and distal structure of centrioles. Singla V, Romaguera-Ros M, Garcia-Verdugo JM, Reiter JF. Dev Cell. 2010 Mar 16. 18(3):410-24. 10.1016/j.devcel.2009.12.022 PubMed 20230748