pIRES2-eGFP-Ofd1-Myc-S75F
(Plasmid #24580)

• Depositing Lab: Jeremy Reiter
• Publication: Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Backbone

• Vector backbone: pIRES2-eGFP
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5578
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ofd1
• Species: M. musculus (mouse)
• Insert Size (bp): 3087
• Mutation: serine 75 changed to phenylalanine (codon TCT changed to TTT)
• GenBank ID: NM_177429
• Entrez Gene: Ofd1 (a.k.a. ORF2, Cxorf5, DXGgc7e)
• Tags / Fusion Proteins:
  • Myc (C terminal on insert)
  • eGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (destroyed during cloning)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: ccctaggtccccagagaca

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mutation reported in:
OFD1, the gene mutated in oral-facial-digital syndrome type 1, is expressed in the metanephros and in human embryonic renal mesenchymal cells.

Romio L, Wright V, Price K, Winyard PJ, Donnai D, Porteous ME, Franco B, Giorgio G, Malcolm S, Woolf AS, Feather SA.

J Am Soc Nephrol. 2003 Mar;14(3):680-9.PMID: 12595504