|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18664||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameRas binding domain of Raf
Insert Size (bp)250
MutationR59A mutation in RBD
/ Fusion Proteins
- mRFP (N terminal on insert)
- mRFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mRFP-RBD(R59A)-mRFP was a gift from Karel Svoboda (Addgene plasmid # 18664 ; http://n2t.net/addgene:18664 ; RRID:Addgene_18664)
For your References section:Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging. Yasuda R, Harvey CD, Zhong H, Sobczyk A, van Aelst L, Svoboda K. Nat Neurosci. 2006 Feb . 9(2):283-91. 10.1038/nn1635 PubMed 16429133