cytoplasmic EKAR (EGFP-mRFP)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18680||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameERK Activity Reporter
Insert Size (bp)550
/ Fusion Proteins
- EGFP (N terminal on insert)
- mRFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:cytoplasmic EKAR (EGFP-mRFP) was a gift from Karel Svoboda (Addgene plasmid # 18680 ; http://n2t.net/addgene:18680 ; RRID:Addgene_18680)
For your References section:A genetically encoded fluorescent sensor of ERK activity. Harvey CD, Ehrhardt AG, Cellurale C, Zhong H, Yasuda R, Davis RJ, Svoboda K.. PNAS 2008 Dec 9;105(49):19264-9 10.1073/pnas.0804598105 PubMed 19033456