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TurboID-MYO10
(Plasmid #245883)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245883 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    EGFPC1
  • Modifications to backbone
    TurboID is replacing the EGFP sequence from the original backbone
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MYO10
  • Species
    H. sapiens (human)
  • Entrez Gene
    MYO10 (a.k.a. MyoX)
  • Tag / Fusion Protein
    • TurboID (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The TurboID-MYO10 plasmid was generated by replacing the EGFP sequence in the EGFPC1-hMyoX plasmid with the TurboID sequence using a gene block. EGFPC1-hMyoX plasmid was a gift from Emanuel Strehler (Addgene plasmid # 47608).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS finds the same Q680R hMyo10 variant as is documented for Addgene plasmid # 47608 (NCBI reference AAF37875.1). Please visit https://doi.org/10.64898/2025.12.03.691809 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TurboID-MYO10 was a gift from Guillaume Jacquemet (Addgene plasmid # 245883 ; http://n2t.net/addgene:245883 ; RRID:Addgene_245883)

  • For your References section:

    CCT8 associates with the MYO10 motor domain and regulates filopodia and breast cancer cell invasion. Popović A, Ojalill M, Touma C, Ball NJ, Miihkinen M, Joshi O, Dibus M, Pylvänäinen J, Ivaska J, Goult BT, Jacquemet G. J Cell Sci jcs.265278 10.1242/jcs.265278
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