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PASTA-5_pUC19_IVT-ePa01
(Plasmid #247041)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247041 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 5154
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ePa01 recombinase (large serine integrase, LSR) for IVT
  • Species
    H. sapiens (human), Synthetic; Pseudomonas aeruginosa, various species of bacteria and bacteriophages

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid can be used for in vitro transcription of enhanced (e)Pa01 mRNA. To do so, a point mutation within the T7 primer needs to be corrected during PCR (forward primer: cgcggccgcTAATACGACTCAC). Also, a poly-A tail needs to be added during PCR (reverse primer: TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTCCTACTCAGGCTTTATTCAAAGACCA). Store mRNA at-80°C and use within 5 min after thawing.

Alternatively, this plasmid can transfected to mammalian cells for CMV-driven expression directly in the target cell, although presumably at reduced editing efficacy and higher toxicity. Please visit https://doi.org/10.1101/2025.09.10.675267 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PASTA-5_pUC19_IVT-ePa01 was a gift from Dimitrios Wagner (Addgene plasmid # 247041 ; http://n2t.net/addgene:247041 ; RRID:Addgene_247041)

  • For your References section:

    Versatile and efficient non-viral integration of large transgenes in human T cells via CRISPR knock-in and engineered integrases. Kassing I, Kath J, Nitulescu A-M, Glaser V, Hartmann LM, Pu Y, Huth L, Kārkliņš R, Shaji S, Ringel AR, Pouzolles M, Stein M, Ibrahim DM, Wagner DL. bioRxiv 2025.09.10.675267 10.1101/2025.09.10.675267
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