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PASTA-4_pUC19_INT-Pa01-attV_P2A_CD19CAR_bGH-pA
(Plasmid #247040)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247040 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 4810
  • Modifications to backbone
    This standard pUC19-backbone has not undergone size-reduction.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pa01 phage attachment site (attP) = vector attachment site (attV), CD19-CAR
  • Species
    H. sapiens (human), Synthetic; sourced from various phage and bacterial genomes

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid resembles PL#1 from our ‘one-pot’ PASTA publication, but utilizes a Pa01 (attP = attV) docking site. This integration-plasmid enables expression of a CD19-CAR through (e)Pa01-mediated in-frame insertion into any translated exon of a transcriptionally active gene (in suitable cell types such as primary human T cells). It is intended for use together with ePa01 mRNA, which can be generated by IVT from our Addgene plasmid #247041 ('PASTA-5_pUC19_IVT-ePa01'). For TRAC integration, generate HDR-template from plasmid ‘PASTA-3_pUC19_HDR-Pa01-attG_TRAC_for-Cas12a’ (Addgene #247039), for Cas12a-assisted HDR-mediated TRAC integration of the corresponding Pa01 landing pad (attB = attG).

A nt discrepancy was found lacZ-alpha that does not affect plasmid function. Please visit https://doi.org/10.1101/2025.09.10.675267 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PASTA-4_pUC19_INT-Pa01-attV_P2A_CD19CAR_bGH-pA was a gift from Dimitrios Wagner (Addgene plasmid # 247040 ; http://n2t.net/addgene:247040 ; RRID:Addgene_247040)

  • For your References section:

    Versatile and efficient non-viral integration of large transgenes in human T cells via CRISPR knock-in and engineered integrases. Kassing I, Kath J, Nitulescu A-M, Glaser V, Hartmann LM, Pu Y, Huth L, Kārkliņš R, Shaji S, Ringel AR, Pouzolles M, Stein M, Ibrahim DM, Wagner DL. bioRxiv 2025.09.10.675267 10.1101/2025.09.10.675267
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