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PiggyBac-hyperdLbCas12a
(Plasmid #247777)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247777 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    PiggyBac-puro
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 12503
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ABE8e-hyperLbCas12a-mCherry-p2a-puro
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5808
  • Promoter EF1a
  • Tag / Fusion Protein
    • mCherry-p2a-puro (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Stanley Qi (the hyperdLbCas12a base editing insert was removed from pSLQ11438, Addgene #183204)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.03.19.644238 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PiggyBac-hyperdLbCas12a was a gift from Aaron McKenna (Addgene plasmid # 247777 ; http://n2t.net/addgene:247777 ; RRID:Addgene_247777)

  • For your References section:

    BASELINE: A CRISPR Base Editing Platform for Mammalian-Scale Single-Cell Lineage Tracing. Winter E, Emiliani F, Cook A, Abderrahim A, McKenna A. bioRxiv 2025.03.19.644238 10.1101/2025.03.19.644238
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