Skip to main content
Addgene
Open Menu

PiggyBac-BASELINE
(Plasmid #247776)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247776 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    PiggyBac-blast
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 14395
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Blasticidin
  • Alt name
    Blast
  • Alt name
    BSD
  • Species
    Synthetic
  • Insert Size (bp)
    399

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use the KpnI site to generate BASELINE recorders with intIDs. Insert oligo used: CTAGACATTGTGCCGCATCAGGTACNNNNNNNNNNNNNNNNNNGTACCAAATCGAGTAACCGTTGCTAGG
Follow NEB's Protocol for Bridging Double-stranded DNA with a Single-stranded DNA oligo.

Please visit https://doi.org/10.1101/2025.03.19.644238 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PiggyBac-BASELINE was a gift from Aaron McKenna (Addgene plasmid # 247776 ; http://n2t.net/addgene:247776 ; RRID:Addgene_247776)

  • For your References section:

    BASELINE: A CRISPR Base Editing Platform for Mammalian-Scale Single-Cell Lineage Tracing. Winter E, Emiliani F, Cook A, Abderrahim A, McKenna A. bioRxiv 2025.03.19.644238 10.1101/2025.03.19.644238
Related items: