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pLEX307-HiBiT-GPNMB-V5
(Plasmid #247980)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247980 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLEX307
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GPNMB
  • Species
    H. sapiens (human)
  • Entrez Gene
    GPNMB (a.k.a. HGFIN, NMB, PLCA3)
  • Promoter EF1A
  • Tags / Fusion Proteins
    • HiBiT (N terminal on insert)
    • V5 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.06.23.661126 for bioRxiv preprint.

The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences. Addgene NGS finds A224T in hGPNMB (NP_001005340.1), and the Kozak sequence deleted upstream of the insert ORF start.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEX307-HiBiT-GPNMB-V5 was a gift from Ellen Sidransky (Addgene plasmid # 247980 ; http://n2t.net/addgene:247980 ; RRID:Addgene_247980)

  • For your References section:

    Bidirectional regulation of glycoprotein nonmetastatic melanoma protein B by beta-glucocerebrosidase deficiency in GBA1 isogenic dopaminergic neurons from a patient with Gaucher disease and parkinsonism. Chen C, Ma C, Sam R, Lichtenberg J, Chen T, Hao Y, Li Z, Kowal I, Andersh K, Qi YA, Perez G, Hertz E, Li Y, Williams D, Henderson MJ, Park M, Jiang X, Jerez PA, Blauwendraat C, Sidransky E, Chen Y. bioRxiv [Preprint]. 2025 Jun 25:2025.06.23.661126. doi: 10.1101/2025.06.23.661126. 10.1101/2025.06.23.661126 PubMed 40667145
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