pZac2.1 gfaABC1D-ARL13B-BioID2-BioID2-HA
(Plasmid
#253927)
-
PurposeExpresses ARL13B-BioID2 in astrocytes
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 253927 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepZac2.1
-
Backbone manufacturerKhakh lab
- Backbone size w/o insert (bp) 5097
- Total vector size (bp) 7920
-
Vector typeMammalian Expression, AAV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameARL13B-BioID2-BioID2-HA
-
Alt nameADP-ribosylation factor-like protein 13B
-
Alt nameBioID2
-
SpeciesH. sapiens (human); Aquifex aeolicus
-
Insert Size (bp)2823
-
GenBank IDNM_001174150
-
Entrez GeneARL13B (a.k.a. ARL2L1, JBTS8)
- Promoter gfaABC1D
-
Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer taatacgactcactatagg
- 3′ sequencing primer gtggtttgtccaaactcatc
- (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byPaul DeCaen; ARL13B was cloned from Addgene plasmid #232880.
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pZac2.1 gfaABC1D-ARL13B-BioID2-BioID2-HA was a gift from Baljit Khakh (Addgene plasmid # 253927 ; http://n2t.net/addgene:253927 ; RRID:Addgene_253927)
