pLenti-Cas12a2-EGFP
(Plasmid #258653)

• Purpose: Expresses mammalian codon-optimized CRISPR-Cas12a2 nuclease (Sulfuricurvum sp. PC08-66) and EGFP under EFS promoter. 3rd-generation lentiviral backbone.
• Depositing Lab: Yang Liu
• Publication: Scholz et al Nature. 2026 May 6. doi: 10.1038/s41586-026-10466-y.

Backbone

• Vector backbone: pFUGW
• Total vector size (bp): 12872
• Vector type: Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Cas12a2
• Alt name: CRISPR-Cas12a2
• Alt name: SuCas12a2
• Species: Sulfuricurvum sp. PC08-66
• Insert Size (bp): 3708
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on insert)

Gene/Insert 2

• Gene/Insert name: EGFP
• Alt name: Enhanced GFP
• Species: Synthetic
• Insert Size (bp): 717
• Tag / Fusion Protein:
  • P2A (N terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry