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pJZ012_EFS-Cas12a2-puro
(Plasmid #257716)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257716 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pJZ012
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SuCas12a2
  • Alt name
    Cas12a2
  • Species
    Sulfuricurvum sp. PC08-66
  • Promoter EF-1alpha
  • Tag / Fusion Protein
    • NLS-FLAG (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.05.08.723607 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJZ012_EFS-Cas12a2-puro was a gift from Jennifer Doudna (Addgene plasmid # 257716 ; http://n2t.net/addgene:257716 ; RRID:Addgene_257716)

  • For your References section:

    Targeting Cancer-Specific Mutations with RNA-Triggered Chromatin Shredding. Zeng J, Cheng Z, Chen H, Wang Z, Thompson J, Crosby KT, Han H, Singhal A, Ngo W, Xia C, Rosas-Rivera D, Zhang Z, Kang MH, Mao Y, Diolaiti ME, Lee GC, Diffley JFX, Song Y, Qiu L, Krah NM, Murthy N, Jackson RN, Liu Y, Ashworth A, Doudna JA. Nature. 2026 Jun 8. doi: 10.1038/s41586-026-10738-7. 10.1038/s41586-026-10738-7 PubMed 42259916
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