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Targeting Cancer-Specific Mutations with RNA-Triggered Chromatin Shredding.

Zeng J, Cheng Z, Chen H, Wang Z, Thompson J, Crosby KT, Han H, Singhal A, Ngo W, Xia C, Rosas-Rivera D, Zhang Z, Kang MH, Mao Y, Diolaiti ME, Lee GC, Diffley JFX, Song Y, Qiu L, Krah NM, Murthy N, Jackson RN, Liu Y, Ashworth A, Doudna JA
Nature. 2026 Jun 8. doi: 10.1038/s41586-026-10738-7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
257716pJZ012_EFS-Cas12a2-puroExpress SuCas12a2 in mammalian cell lines; Puromycin selection.
257717pJZ015_EFS-Cas12a2-TagBFPExpress SuCas12a2 in mammalian cell lines; TagBFP marker.
257718plx313-EGFR_E746_A750delExpress EGFR E746_A750 in-frame deletion mutant in mammalian cells; Geneticin/G418 selection
257719plx313-EGFRwtExpress EGFR WT in mammalian cells; Geneticin/G418 selection
257720plx313-tp53-e285kExpress TP53 E285K mutant in mammalian cells; Geneticin/G418 selection
257721plx313-tp53-r175hExpress TP53 R175H mutant in mammalian cells; Geneticin/G418 selection
257722plx313-tp53-r248qExpress TP53 R248Q mutant in mammalian cells; Geneticin/G418 selection
257723plx313-tp53-r280kExpress TP53 R280K mutant in mammalian cells; Geneticin/G418 selection

Antibodies from Article