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pET15-b_holD-GS8-holC
(Plasmid #254006)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254006 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET15b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    holD-GS8-holC (ψ-GS8-χ fusion)
  • Species
    E. coli

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15-b_holD-GS8-holC was a gift from Linda Bloom (Addgene plasmid # 254006 ; http://n2t.net/addgene:254006 ; RRID:Addgene_254006)

  • For your References section:

    A novel ψ-χ fusion protein for unravelling the contributions of χ to DNA replication and repair. Padgett-Pagliai KA, Wimer E, Grant JD, Petrides MJ, Newcomb ESP, Sutera VA, Lovett ST, Bloom LB. Nucleic Acids Res. 2026 May 20;54(10):gkag533. doi: 10.1093/nar/gkag533. 10.1093/nar/gkag533 PubMed 42200296
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