A novel ψ-χ fusion protein for unravelling the contributions of χ to DNA replication and repair.

Padgett-Pagliai KA, Wimer E, Grant JD, Petrides MJ, Newcomb ESP, Sutera VA, Lovett ST, Bloom LB
Nucleic Acids Res. 2026 May 20;54(10):gkag533. doi: 10.1093/nar/gkag533. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
254005	pET15-b_holD-GS12-holC	T7 expression vector encoding E. coli ψ-GS12-χ fusion for biochemical reconstitution and clamp loader studies.
254006	pET15-b_holD-GS8-holC	T7 expression vector encoding E. coli ψ-GS8-χ fusion for biochemical and functional clamp loader assays.
254007	pETDuet-1_holB	T7 co-expression vector encoding E. coli holB (δ′) for clamp loader complex reconstitution.
254008	pETDuet-1_holD-GS8-holC_holB	T7 co-expression vector encoding ψ-GS8-χ fusion (MCS1) and holB (MCS2) for clamp loader assembly studies.
254009	pETDuet-1_holD-GS12-holC_holB	T7 co-expression vector encoding ψ-GS12-χ fusion (MCS1) and holB (MCS2) for clamp loader reconstitution.
254010	pCOLADuet-1_holA_dnaX	T7 co-expression vector encoding holA (δ) and dnaX (γ) for E. coli clamp loader complex assembly.
254011	pUC57_holD-GS12-holC	Cloning vector containing synthetic ψ-GS12-χ fusion insert for subcloning and construct generation.
254013	pBAD18_holD	Arabinose-inducible vector expressing E. coli holD (ψ) for in vivo complementation studies.
254014	pBAD18_holC	Arabinose-inducible vector expressing E. coli holC (χ) for complementation and functional assays.
254015	pBAD18_holD_holC	Arabinose-inducible bicistronic vector expressing holD and holC for clamp loader complementation.
254016	pBAD18_holD-GS12-holC	Arabinose-inducible vector expressing ψ-GS12-χ fusion for in vivo functional analysis.
254017	pBAD18_holD-GS12-holC_R128A	Arabinose-inducible ψ-GS12-χ fusion containing χ R128A mutation disrupting SSB interaction.