pCOLADuet-1_holA_dnaX
(Plasmid
#254010)
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PurposeT7 co-expression vector encoding holA (δ) and dnaX (γ) for E. coli clamp loader complex assembly.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254010 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCOLADuet1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameholA + dnaX
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SpeciesE. coli
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unspecified (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCOLADuet-1_holA_dnaX was a gift from Linda Bloom (Addgene plasmid # 254010 ; http://n2t.net/addgene:254010 ; RRID:Addgene_254010) -
For your References section:
A novel ψ-χ fusion protein for unravelling the contributions of χ to DNA replication and repair. Padgett-Pagliai KA, Wimer E, Grant JD, Petrides MJ, Newcomb ESP, Sutera VA, Lovett ST, Bloom LB. Nucleic Acids Res. 2026 May 20;54(10):gkag533. doi: 10.1093/nar/gkag533. 10.1093/nar/gkag533 PubMed 42200296
