pBAD18_holD-GS12-holC
(Plasmid
#254016)
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PurposeArabinose-inducible vector expressing ψ-GS12-χ fusion for in vivo functional analysis.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254016 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepBAD18
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameholD-GS12-holC
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SpeciesE. coli
Cloning Information
- Cloning method Gene Synthesis
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD18_holD-GS12-holC was a gift from Linda Bloom (Addgene plasmid # 254016 ; http://n2t.net/addgene:254016 ; RRID:Addgene_254016) -
For your References section:
A novel ψ-χ fusion protein for unravelling the contributions of χ to DNA replication and repair. Padgett-Pagliai KA, Wimer E, Grant JD, Petrides MJ, Newcomb ESP, Sutera VA, Lovett ST, Bloom LB. Nucleic Acids Res. 2026 May 20;54(10):gkag533. doi: 10.1093/nar/gkag533. 10.1093/nar/gkag533 PubMed 42200296