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pMUT1-pTlpA-csgAwt
(Plasmid #254238)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254238 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pMUT1
  • Backbone manufacturer
    Native to E. coli Nissle 1917
  • Total vector size (bp) 9965
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Mach1
  • Growth instructions
    Mach1 E. coli may also be used for transformation
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Synthetic curli operon with temperature-sensitive (TlpA) regulation
  • Species
    Synthetic
  • Insert Size (bp)
    4733
  • Promoter pTlpA

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer cattactcgcatccattctcaggc
  • 3′ sequencing primer cctgggattgcagccgcagcaggt
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMUT1-pTlpA-csgAwt was a gift from Neel Joshi (Addgene plasmid # 254238 ; http://n2t.net/addgene:254238 ; RRID:Addgene_254238)
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