Neel Joshi Lab: Modulating Innate Immune Responses to Curli Fibers Through Protein Engineering

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
254238	pMUT1-pTlpA-csgAwt	pMUT1 vector native to E. coli Nissle 1917 with ampicillin resistance and temperature sensitive wild-type curli expression
254239	pMUT1-pTlpA-csgA-SSL3	pMUT1 vector native to E. coli Nissle 1917 with ampicillin resistance and temperature sensitive curli expression. CsgA has been modified with a C-terminal fusion encoding SSL3.
254240	pMUT1-pTlpA-csgA-S6E11	pMUT1 vector native to E. coli Nissle 1917 with ampicillin resistance and temperature sensitive curli expression. CsgA has been modified with a C-terminal fusion encoding SELP (S6E11).
254241	pET21d-csgAwt	pET21d synthetic vector containing csgA and csgC for expression. csgA has been modified by deletion of the N-terminal Sec signal peptide to enable cytosolic curli production for purification.
254242	pET21d-csgA-SSL3	pET21d synthetic vector containing csgA-SSL3 and csgC for expression. csgA has been modified by deletion of the N-terminal Sec signal peptide to enable cytosolic curli production for purification.
254243	pET21d-csgA-S6E11	pET21d synthetic vector containing csgA-S6E11 and csgC for expression. csgA has been modified by deletion of the N-terminal Sec signal peptide to enable cytosolic curli production for purification.
254244	pET21d-sSSL3	pET21d synthetic vector containing SSL3 for cytosolic expression.