pMUT1-pTlpA-csgA-S6E11
(Plasmid
#254240)
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PurposepMUT1 vector native to E. coli Nissle 1917 with ampicillin resistance and temperature sensitive curli expression. CsgA has been modified with a C-terminal fusion encoding SELP (S6E11).
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Depositing Lab
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Depositing OrganizationNortheastern University
Located in the United States of America -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254240 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepMUT1
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Backbone manufacturerNative to E. coli Nissle 1917
- Total vector size (bp) 10295
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Mach1
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Growth instructionsMach1 E. coli may also be used for transformation
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSynthetic curli operon with temperature-sensitive (TlpA) regulation; csgA fused to S6E11
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SpeciesSynthetic
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Insert Size (bp)5063
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMUT1-pTlpA-csgA-S6E11 was a gift from Neel Joshi (Addgene plasmid # 254240 ; http://n2t.net/addgene:254240 ; RRID:Addgene_254240)
Map uploaded by the depositor.
