pET21d-csgAwt
(Plasmid
#254241)
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PurposepET21d synthetic vector containing csgA and csgC for expression. csgA has been modified by deletion of the N-terminal Sec signal peptide to enable cytosolic curli production for purification.
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Depositing Lab
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Depositing OrganizationNortheastern University
Located in United States of America -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254241 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepET21d
- Total vector size (bp) 6168
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsMach1 E. coli may also be used for transformation
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namecsgA
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SpeciesSynthetic
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Insert Size (bp)453
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET21d-csgAwt was a gift from Neel Joshi (Addgene plasmid # 254241 ; http://n2t.net/addgene:254241 ; RRID:Addgene_254241)
Map uploaded by the depositor.
