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pMSL88
(Plasmid #254505)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254505 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRSFDuet™-1
  • Backbone manufacturer
    SnapGene
  • Backbone size w/o insert (bp) 3829
  • Total vector size (bp) 9588
  • Modifications to backbone
    MCS-1: Cas6, Cas8, Cas7, Cas5 MCS-2: Repeat-Spacer-Repeat, CasDinG-HNH
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Cas6
  • Alt name
    csf5
  • Species
    Pseudomonas oleovorans
  • Insert Size (bp)
    711
  • GenBank ID
  • Promoter T7

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Cas8
  • Alt name
    csf1
  • Species
    Pseudomonas oleovorans
  • Insert Size (bp)
    724
  • GenBank ID
  • Promoter T7

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    Cas7
  • Alt name
    csf2
  • Species
    Pseudomonas oleovorans
  • Insert Size (bp)
    1043
  • GenBank ID
  • Promoter T7

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    Cas5
  • Alt name
    csf3
  • Insert Size (bp)
    668
  • GenBank ID
  • Promoter T7

Cloning Information for Gene/Insert 4

Gene/Insert 5

  • Gene/Insert name
    CRISPR-RNA
  • Alt name
    Repeat-Spacer-Repeat
  • Species
    Pseudomonas oleovorans
  • Insert Size (bp)
    90
  • GenBank ID
  • Promoter T7

Cloning Information for Gene/Insert 5

Gene/Insert 6

  • Gene/Insert name
    CasDinG-HNH
  • Alt name
    csf4
  • Species
    Pseudomonas oleovorans
  • Insert Size (bp)
    2493
  • GenBank ID
  • Promoter T7
  • Tag / Fusion Protein
    • HNH domain (C terminal on insert)

Cloning Information for Gene/Insert 6

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

All-in-one plasmid expressing:
- Type IV-AI Cas5,6,7,8
- Repeat-Spacer-Repeat CRISPR-RNA (Filler spacer can be exchanged by BseRI golden gate cloning for desired target RNA)
- CasDinG-HNH

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSL88 was a gift from Lennart Randau (Addgene plasmid # 254505 ; http://n2t.net/addgene:254505 ; RRID:Addgene_254505)

  • For your References section:

    Type I-Fv and engineered type IV-A1 CRISPR-Cas effectors facilitate genome reduction in Escherichia coli. Klein N, Sanchez-Londono M, Kara MM, Gomes-Filho JV, Novak S, Kholeif KH, Pekarek L, Caliskan N, Randau L. Nucleic Acids Res. 2025 Nov 26;53(22):gkaf1399. doi: 10.1093/nar/gkaf1399. 10.1093/nar/gkaf1399 PubMed 41414667
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