Type I-Fv and engineered type IV-A1 CRISPR-Cas effectors facilitate genome reduction in Escherichia coli.

Klein N, Sanchez-Londono M, Kara MM, Gomes-Filho JV, Novak S, Kholeif KH, Pekarek L, Caliskan N, Randau L
Nucleic Acids Res. 2025 Nov 26;53(22):gkaf1399. doi: 10.1093/nar/gkaf1399. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
254505	pMSL88	Expresses Type IV-A1 Cas5,6,7,8, Repeat-Spacer-Repeat CRISPR-RNA, and CasDinG-HNH from Pseudomonas oleovorans. CasDinG was engineered to include a C-terminal HNH nuclease domain