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pICE1Sc
(Plasmid #254930)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254930 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    p15A
  • Vector type
    CRISPR, Synthetic Biology
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    dCas9, divIVA spacer
  • gRNA/shRNA sequence
    cctcgtcatagccttctcgg
  • Insert Size (bp)
    4107

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pICE1Sc was a gift from Gilles van Wezel (Addgene plasmid # 254930 ; http://n2t.net/addgene:254930 ; RRID:Addgene_254930)

  • For your References section:

    Inducible CRISPRi enables efficient and high-fidelity genome editing in Streptomyces. Bai C, Zhu H, Bayona LM, Du C, van Wezel GP. Nucleic Acids Res. 2026 Jun 8;54(11):gkag560. doi: 10.1093/nar/gkag560. 10.1093/nar/gkag560 PubMed 42258544
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