Skip to main content
Addgene
Open Menu

pLV hU6-sgNT2-2-hUbC-dCas9-KRAB-T2a-Puro
(Plasmid #255340)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255340 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro
  • Backbone manufacturer
    Charles Gersbach (Addgene #71236)
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    sgNT2
  • gRNA/shRNA sequence
    gctgcatggggcgcgaatca
  • Species
    Synthetic

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV hU6-sgNT2-2-hUbC-dCas9-KRAB-T2a-Puro was a gift from Kenneth Chen (Addgene plasmid # 255340 ; http://n2t.net/addgene:255340 ; RRID:Addgene_255340)

  • For your References section:

    DROSHA Regulates Mesenchymal Gene Expression in Wilms Tumor. Tiburcio PDB, Desai K, Kim J, Zhou Q, Guo L, Xiao X, Zhou L, Yuksel A, Catchpoole DR, Amatruda JF, Xu L, Chen KS. Mol Cancer Res. 2024 Aug 2;22(8):711-720. doi: 10.1158/1541-7786.MCR-23-0930. 10.1158/1541-7786.MCR-23-0930 PubMed 38647377
Related items: