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Sp6-PE2-S525A
(Plasmid #255520)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255520 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCMV-Sport 6
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4396
  • Total vector size (bp) 5419
  • Modifications to backbone
    Introduction of Igk leader
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pre-thrombin 2 S525A
  • Alt name
    PE2 S525A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    924
  • Mutation
    Deleted from M1 till R271and changed Serine 525 to Alanine
  • GenBank ID
    NM_000506.5
  • Entrez Gene
    F2 (a.k.a. PT, RPRGL2, THPH1)
  • Promoter CMV
  • Tag / Fusion Protein
    • His-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BstEII (not destroyed)
  • 3′ cloning site AsiSI (not destroyed)
  • 5′ sequencing primer CAGGAAACAGCTATGAC
  • 3′ sequencing primer GTAAAACGACGGCCAGT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Sp6-PE2-S525A was a gift from F Xavier Gomis-Ruth (Addgene plasmid # 255520 ; http://n2t.net/addgene:255520 ; RRID:Addgene_255520)

  • For your References section:

    A human-cell recombinant prethrombin-2 platform producing thrombin with enhanced fibrinogen processing for in vitro applications. Swaid H, Mendes SR, Fuentes-Prior P, Rodriguez-Banqueri A, Gomis-Ruth FX. Thromb Res. 2026 Oct 5;266:109863. doi: 10.1016/j.thromres.2026.109863. 10.1016/j.thromres.2026.109863 PubMed 42837787
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