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pLVX-TetOne-Puro-GFP-3xFLAG
(Plasmid #258081)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258081 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLVX-TetOne-Puro
  • Backbone manufacturer
    Clontech
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    792
  • Promoter TRE3GS
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site AgeI (unknown if destroyed)
  • 5′ sequencing primer AACGGACGTGAAGAATGTG
  • 3′ sequencing primer TCACTCTCTTACCCGTCATT
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-TetOne-Puro-GFP-3xFLAG was a gift from Alexis Jourdain (Addgene plasmid # 258081 ; http://n2t.net/addgene:258081 ; RRID:Addgene_258081)

  • For your References section:

    Functional nutrient-genetic profiling reveals biotin and FBXW7 are essential to bypass glutamine addiction. Lisci M, Vericel F, Liu Y, Gallart-Ayala H, Ivanisevic J, Skinner OS, Jourdain AA. Mol Cell. 2026 Mar 5;86(5):901-916.e10. doi: 10.1016/j.molcel.2026.02.002. Epub 2026 Feb 25. 10.1016/j.molcel.2026.02.002 PubMed 41747732
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