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Alexis Jourdain Lab: Functional nutrient-genetic profiling reveals biotin and FBXW7 are essential to bypass glutamine addiction.

Lisci M, Vericel F, Liu Y, Gallart-Ayala H, Ivanisevic J, Skinner OS, Jourdain AA
Mol Cell. 2026 Mar 5;86(5):901-916.e10. doi: 10.1016/j.molcel.2026.02.002. Epub 2026 Feb 25. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
258081pLVX-TetOne-Puro-GFP-3xFLAGOverexpression
258082pTwist-Lenti-CMV-BSD-GFP-3xFLAGOverexpression
258083pTwist-Lenti-CMV-BSD-PC-WT-3xFLAGOverexpression
258084pTwist-Lenti-CMV-BSD-PC-K1144R-3xFLAGOverexpression
258085pTwist-Lenti-CMV-Puro-GFP-3xFLAGOverexpression
258086pTwist-Lenti-CMV-Puro-FBXW7-WTOverexpression
258087pTwist-Lenti-CMV-Puro-FBXW7-R465COverexpression
258088pTwist-Lenti-CMV-Puro-FBXW7-R505COverexpression
258089pTwist-Lenti-CMV-Puro-FBXW7-R479QOverexpression
258090pLentiCRISPR v2 MDH1 sg1Knockout
258093pLentiCRISPR v2 GOT1 sg2Knockout
258094pLentiCRISPR v2 SLC5A6 sg1Knockout
258095pLentiCRISPR v2 SLC5A6 sg2Knockout
258096pLentiCRISPR v2 HLCS sg1Knockout
258097pLentiCRISPR v2 HLCS sg2Knockout
258100pLentiCRISPR v2 FBXW7 sg1Knockout
258101pLentiCRISPR v2 FBXW7 sg2Knockout
258103pLentiCRISPR v2 MNT sg2Knockout
258104pLentiCRISPR v2 SIN3A sg1Knockout
258105pLentiCRISPR v2 SIN3A sg2Knockout
258106pLentiCRISPR v2 MLX sg1Knockout
258107pLentiCRISPR v2 MLX sg2Knockout
258108pLentiCRISPR v2 MLXIP sg1Knockout
258109pLentiCRISPR v2 MLXIP sg2Knockout

Antibodies from Article